Part:BBa_K1614015:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1614015
Along with the ATP Aptamer JAWS1 Spinach2 (BBa_K1614014), ATP Aptamer JAWS2 Spinach2 (BBa_K1614015) was generated by the software JAWS that was designed by the iGEM Team Heidelberg 2015. The ATP Aptamer JAWS2 Spinach2 was transcribed using T7 RNA Polymerase and purified by denaturing polyacrylamide gel electrophoresis. Spectra have shown that the ATP Aptamer JAWS2 Spinach2 has the highest peak of all tested ATP Aptamer Spinach2 variations. This ATP dependent Spinach2 was designed for the detection of ATP in dynamic biochemical reactions, but its strong stemming does not allow the sensing of ATP in dynamic processes. Hence, sensor can be probably applied for in vivo imaging experiments.
User Reviews
UNIQ2379a76cf6b46bdd-partinfo-00000000-QINU UNIQ2379a76cf6b46bdd-partinfo-00000001-QINU